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KMID : 1190620100060020069
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Journal of the Korean Wound Care Society
2010 Volume.6 No. 2 p.69 ~ p.76
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Effect of rrhGM-CSF on Wound Healing in Hamster Model of Burn Trauma
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Heo Si-Hyun
Kim Ji-Hyun
Yoon Kwang-Ho
Lee Young-Jun
Han Kyu-Boem
Kim Wan-Jong
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Abstract
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A burn is one of the most common and devastating forms of trauma. A number of approaches were taken in burn repair. GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rrhGM-CSF, which contains more oligosaccharide side chains than any other types of GM-CSF, was specifically produced from rice cell culture in our laboratory (CHA Bio & Diostech Co., Ltd.). The present study was taken to evaluate the influence on burn (2nd degree) wound healing of rrhGM-CSF in male golden hamsters (Mesocricetus auratus). Methodologically, deep second-degree burn defects of 1.5 cm in diameter were made using a hot iron in the back skin of hamsters, and 100 ?l base cream containing rrhGM-CSF 50 ?g/ml (experimental groups) was applied. Control groups were given only base cream without rrhGM-CSF. The wound sizes of experimental groups were macroscopically reduced from 6 days after burn compared to those of control group and almost repaired by 15 days. Light microscopically, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. Immunohistochemically, positive reaction of PCNA which indicates cell proliferation in the skin following injury was more prominent in the experimental group. Since this type of GM-CSF is highly glycosylated, the effectiveness might be retain longer and stable during wound healing in the animal model. The results suggest that rrhGM-CSF can be used for further development of the therapeutic manipulation of burn wound healing. (J Korean Wound Management Soc 2010;6:69-76)
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KEYWORD
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Deep second-degree burn, rrhGM-CSF, Burn wound healing, Hamster
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